|
Hematopathology |
|
|
TEST NAME & TAT |
CLINICAL CONDITION |
SPECIMEN TYPE |
COLLECTION |
PROCESSING |
SHIPPING |
|
|
Major t (9;22) quantitative
by Real-Time PCR (14 days) |
Determination of t(9;22)
translocation (Philadelphia chromosome) in CML patients and monitoring |
Bone
marrow or peripheral blood |
Collect
1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Keep at
room temp |
Ship
overnight at room temperature |
|
|
Quantitation of e1a2 bcr-abl
transcripts (Minor Breakpoint Region) by Real-Time PCR (14 days) |
Confirm suspected diagnosis of
Acute Lymphoblastic Leukemia; monitoring of minimal residual disease (with
chemotherapies and after bone marrow transplantation |
Bone
marrow or peripheral blood |
Collect
1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Keep at
room temp |
Ship
overnight at room temperature |
|
|
Engraftment Baseline (10 days after receipt of
Follow-up specimen) |
Determine recipient genotype
prior to bone marrow transplant |
Bone
marrow or peripheral blood |
Collect
1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate
sample 4° C until shipped |
Ship on
cold packs to maintain temperature |
|
|
Buccal
swab |
Swab
inner facial cheek |
Seal in
swab storage envelope and keep at room temp |
Ship at
room temperature |
|
|
Engraftment Donor (10 days after receipt of
Follow-up specimen) |
Determine donor genotype prior to
bone marrow transplant |
Bone
marrow or peripheral blood |
Collect
1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate
sample 4° C until shipped |
Ship on
cold packs to maintain temperature |
|
|
Buccal
swab |
Swab
inner facial cheek |
Seal in
swab storage envelope and keep at room temp |
Ship at
room temperature |
|
Engraftment
Follow-up (10days)
|
Monitoring
for chimerism state following bone marrow engraftment
|
Bone
marrow or peripheral blood
|
Collect
1.0mL of BM or 3.0 mL of PB in EDTA (purple-top) tube
|
Refrigerate
sample 4° C until shipped
|
Ship on cold packs to maintain temperature
|
|
T-Cell Engraftment
Follow-up (10days)
|
Monitoring
for chimerism state following bone marrow engraftment
using isolated T cells |
Peripheral blood
|
Collect 3.0 mL of PB in EDTA (purple-top) tube. Samples should only be collected Monday – Thursday.
|
Refrigerate
sample 4° C until shipped. T cells must be isolated within 24 hours of collection.
|
Ship on cold packs to maintain temperature
|
|
|
JAK2 by PCR (14 days)
|
Confirm diagnosis of Polycythemia Vera in patients with increased red cell mass and a low serum erythropoietin level
|
Peripheral blood
|
Collect 3.0 mL of PB in EDTA (purple-top) tube
|
Refrigerate sample at 4° C until shipped
|
Ship on cold packs to maintain temperature |
|
|
B &
T-cell clonality by PCR (14 days) |
Determination
of clonality of B and/or T cells in lymphomas and leukemias |
Bone
marrow or peripheral blood |
Collect
1.0mL of BM or 3.0 mL of PB in EDTA (purple top) or ACD (yellow-top) tube |
Refrigerate
sample 4° C until shipped |
Ship on
cold packs to maintain temperature |
|
|
Frozen
tissue |
Biopsy
or excise sample |
Snap
freeze and hold in liquid nitrogen |
Ship on
dry ice |
|
|
t (14;18) translocation by PCR
(21 days) |
Determination
of t(14;18) translocation in B-cell lymphoma; diagnosis of follicular
lymphoma, minimal residual disease |
|
|
Paraffin
section |
None |
Cut 2-6
ribbons of 15-20 sections 5 ΅m thick; roll up and place into screw-top
tubes provided by MDx lab |
Ship in
screw top tubes provided by MDx lab |
|
|
T&B-cell
gene rearrangement by Southern blot hybridization analysis (25 days) |
Determination
of monoclonal rearrangement of B-cell IgH or T-cell surface receptors |
Frozen
tissue |
Biopsy
or excised sample |
Snap
freeze and hold in liquid nitrogen |
Ship on dry ice |
|
|
Bone marrow or peripheral blood |
Collect 1.0 mL of BM or 3.0 mL of
PB in EDTA (purple-top) or ACD (yellow-top) tube |
Refrigerate sample at 4° C |
Ship on cold packs to maintain
temperature |
|
|
Infectious Diseases
|
|
TEST NAME & TAT
|
CLINICAL CONDITION
|
SPECIMEN TYPE
|
COLLECTION
|
PROCESSING
|
SHIPPING
|
|
BK Virus
using real-time PCR (14 days)
|
Follow-up
to kidney transplant
|
Urine or
peripheral blood
|
10mL
plastic tube (no additives) of urine; and/or 3.0 mL EDTA (purple-top) tube
of peripheral blood
|
For urine
|
Deliver
immediately (within 30 minutes) to lab so that the urine and/or plasma can
be frozen until analysis
|
|
CMV
quantitative (7 days)
|
Monitoring
of CMV viremia in immunocompromised patients
Diagnosis of CMV
disease
|
Peripheral
blood
|
Collect 3.0 mL of PB in
EDTA (purple-top) or PPT (pearl-top) tube
|
Spin
down and pipet off plasma within 4 hrs of collection; place plasma in
freezer at 20° C
|
Ship
frozen on dry ice
|
|
Epstein
Barr Virus (EBV) Quantitative by PCR
(10
days)
|
Diagnosis
and monitoring of patients with Post-Transplant Lymphoproliferative
Disorder (PTLD)
|
Peripheral
blood
|
Collect 3.0 mL of PB in
EDTA (purple-top) or PPT (pearl-top) tube
|
Spin
down and pipet off plasma within 4 hrs of collection; place plasma in
freezer at 20° C
|
Ship
frozen on dry ice
|
|
HBV
quantitative by PCR
(21
days)
|
Determine
ongoing versus resolved HBV infection; monitor response to antiviral
therapy
|
Peripheral
blood
|
Collect 3.0 mL of PB in
EDTA (purple-top) or PPT (pearl-top) tube
|
Spin
down and pipet off plasma within 4 hrs of collection; place plasma in
freezer at 20° C
|
Ship
frozen on dry ice
|
|
HCV quantitative by PCR
(14 days)
|
Monitoring of viremia in patients diagnosed with HCV
infection and determination of genotype
|
Peripheral blood
|
Collect 3.0 mL of PB in EDTA (purple-top) or PPT
(pearl-top) tube
|
Spin down and pipet off plasma within 4 hrs of
collection; place serum in freezer at 20° C
|
Ship frozen on dry ice; Contact 828-7284 for pickup or
shipping details
|
|
HCV genotyping (17 days)
|
|
HIV
quantitative & HIV Ultrasensitive quantitative by PCR
(14
days)
|
Monitoring
of viremia in patients diagnosed with HIV infection and identification of
antiretroviral drug resistance mutations
|
Peripheral
blood
|
Collect
3.0 mL of PB in EDTA (purple-top) or PPT (pearl-top) tube
|
Spin
down and pipet off plasma within 4 hrs of collection; place plasma in
freezer at 20° C
|
Ship
frozen on dry ice; Contact 828-7284 for pickup or shipping details
|
|
HIV genotyping (14 days)
|
|
Herpes Simplex Virus (HSV) in
Cerebrospinal fluid (CSF) (7 days)
|
Diagnosis and monitoring in
neuropathy when HSV is suspected cause
|
CSF
|
A separate tube of CSF should be
drawn if at all possible to prevent contamination of the sample
|
|
Deliver immediately to lab so
that it can be frozen until analysis
|
|
Inherited Diseases |
|
TEST NAME & TAT |
CLINICAL CONDITION |
SPECIMEN TYPE |
COLLECTION |
PROCESSING |
SHIPPING |
|
CF
Carrier Screening
(14
days) |
Identify
inherited cause of Cystic Fibrosis that affects the respiratory tract,
pancreas, intestines, reproductive hepatobiliary system and abnormally
thick mucus that could lead to pneumonia, diarrhea, poor growth and
infertility. |
Peripheral
blood |
Collect
3.0 mL of PB in EDTA (purple-top tube) |
Refrigerate
sample at 4° C until shipped |
Ship on
cold packs to maintain temperature |
|
Factor V
Leiden (10 days)
|
Determine
cause of familial or personal history of hypercoagulability |
Peripheral
blood |
Collect
1 full citrate (blue top) or EDTA (purple top) tube of peripheral
blood |
Refrigerate
sample at 4° C until shipped |
Ship on
cold packs to maintain temperature |
|
Fragile X testing
(21
days) |
Determine
fragile X gene mutation as cause or potential cause of mental retardation |
Peripheral
blood |
Collect
3.0 mL of PB in EDTA (purple-top tube) |
Refrigerate
sample at 4° C until shipped |
Ship on
cold packs to maintain temperature |
|
Hereditary
pancreatitis
(21
days)
|
Identify
inherited cause of pancreatitis |
Peripheral blood |
Collect 3.0 mL of PB in EDTA
(purple-top) tube |
Refrigerate sample at 4° C until
shipped |
Ship on cold packs to maintain
temperature |
|
Prader-Willi/Angelman
syndrome
(21 days)
|
Identify
inherited cause of mental retardation |
Peripheral blood |
Collect
3.0 mL of PB in EDTA (purple-top) tube |
Refrigerate
sample at 4° C until shipped |
Ship on
cold packs to maintain temperature |
|
Prothrombin
G20210A
(10 days)
|
Determine
cause of familial or personal history of hypercoagulability |
Peripheral
blood |
Collect
1 full citrate (blue top) or EDTA (purple top) tube of peripheral
blood |
Refrigerate
sample at 4° C until shipped |
Ship on
cold packs to maintain temperature |
